Journal: Cancer Biology & Therapy

Article Title: Transcription factor 3 promotes migration and invasion potential and maintains cancer stemness by activating ID1 expression in esophageal squamous cell carcinoma

doi: 10.1080/15384047.2023.2246206

Figure Lengend Snippet: TCF3 regulates the expression of cancer stem markers CD44 and CD133 by transcriptionally regulating ID1. a. RNA-seq results show significantly upregulated and downregulated gene signatures. b-c. In KYSE-150 and TE-1, with the knockdown of TCF3 the protein expression level of ID1 is subsequently reduced. d-e. In KYSE-150 and TE-1, with the knockdown of TCF3, the mRNA expression of ID1 is subsequently reduced. f-g. CHIP and Dual luciferase reporter assay suggested TCF3 could transcriptionally regulate ID1.h-i. With knockdown of TCF3 in KYSE-150 and TE-1 CD44, fluorescence intensity decreased. J-K. The protein expression of CD44 and CD133 was reduced with the knockdown of TCF3 in KYSE-150 and TE-1. l -m. With the knockdown of TCF3 in KYSE-150 and TE-1 knockdown increased sensitivity to the chemotherapeutic drug cisplatin. n-o. mRNA expression of ID1 was reduced in KYSE-150 and TE-1 after siRNA knockdown. p-q. The protein expression level of CD44 and CD133 was subsequently reduced in KYSE-150 and TE-1 when ID1 was knocked down. I. KYSE-150 has a significant decrease in the sphere formation efficiency after si-TCF3 was transfected. s. with knockdown of TCF3, CD44+ ESCC cells number were decreased.

Article Snippet: CD44 antibody purchased from R&DSystems (FAB6127G).

Techniques: Expressing, RNA Sequencing Assay, Luciferase, Reporter Assay, Fluorescence, Transfection

Journal: Cancer Biology & Therapy

Article Title: Transcription factor 3 promotes migration and invasion potential and maintains cancer stemness by activating ID1 expression in esophageal squamous cell carcinoma

doi: 10.1080/15384047.2023.2246206

Figure Lengend Snippet: ID1 promotes proliferation, migration, invasion, and drug resistance of ESCC cells. a-b. The fluorescence intensity of ID1 was reduced in KYSE-150 and TE-1 after ID1 was knocked down. c-d. The migration and invasion abilities of KYSE-150 and TE-1 were decreased when ID1 was knocked down. e -f. The wound healing rates of KYSE-150 and TE-1 were decreased when ID1 was knocked down. g-h. Cell proliferation is affected in KYSE-150 and TE-1 after the siRNA knockdown of ID1.I&J. Increased sensitivity to the chemotherapeutic drug cisplatin with knockdown of TCF3 in KYSE-150 and TE-1. k. KYSE150 has a significant decrease in the sphere formation efficiency after si-ID1 was transfected. l. with knockdown of ID1, CD44+ ESCC cells number were decreased.

Article Snippet: CD44 antibody purchased from R&DSystems (FAB6127G).

Techniques: Migration, Fluorescence, Transfection

Journal: Cancer Biology & Therapy

Article Title: Transcription factor 3 promotes migration and invasion potential and maintains cancer stemness by activating ID1 expression in esophageal squamous cell carcinoma

doi: 10.1080/15384047.2023.2246206

Figure Lengend Snippet: The expressions of cancer stem markers CD44 and CD133 were correlated with the progression of ESCC. a. The expression of CD44 is corresponding to the tumor staging of ESCC. b. The expression of CD133 is corresponding to the tumor staging of ESCC. c. The expression of CD44 positively correlates with TCF3 expression.d.CD133 positively correlates with the expression of TCF3. * p < .05, ** p < .01. *** p < .001.

Article Snippet: CD44 antibody purchased from R&DSystems (FAB6127G).

Techniques: Expressing

Journal: Journal of tissue engineering and regenerative medicine

Article Title: Persistence of fluorescent nanoparticle-labeled bone marrow mesenchymal stem cells in vitro and after intra-articular injection

doi: 10.1002/term.2781

Figure Lengend Snippet: Cell surface markers of unlabeled and QD-labeled MSCs determined by flow cytometry.

Article Snippet: Control and QD-MSCs at P3 were immunophenotyped by assessing immunoreaction using mouse anti-horse antibodies to MHCII (Bio-Rad, Raleigh, NC), CD44 (Bio-Rad), CD29 (Beckman Coulter, Brea, CA), CD90 (VMRD Inc., Pullman, WA), and CD45RB (VMRD Inc.) as previously described ( Mitchell et al. , 2015 ).

Techniques: Cytometry

Journal: International Journal of Molecular Medicine

Article Title: Effects of cisplatin on the proliferation, invasion and apoptosis of breast cancer cells following β-catenin silencing

doi: 10.3892/ijmm.2020.4543

Figure Lengend Snippet: Expression levels of CD44/54 in BC cells are analyzed by flow cytometry. (A) T47D cells treated with cisplatin, siR-β-catenin and the combination of cisplatin and siR-β-catenin were analyzed by FACS and the fluorescence intensities of CD44/CD54 were obtained. (B) Statistical analysis of the expression of CD44/CD54 in T47D cells. (C) The expression of CD44/CD54 in MCF-7 cells treated with cisplatin, siR-β-catenin and the combination of cisplatin and siR-β-catenin were analyzed by FACS. (D) Statistical analysis of the expression of CD44/CD54 in MCF-7 cells. All data are presented as mean ± standard error of the mean. * P<0.05, ** P<0.01 and *** P<0.001 vs. control. BC, breast cancer; siRNA; small interfering RNA; CD44, CD44 antigen; CD54, intercellular adhesion molecule 1.

Article Snippet: The cells were stained with phycoerythrin-labeled mono-clonal CD44 (cat. no. MAB6127; 1:200; R&D Systems, Inc.) or allophycocyanin-labeled CD54 (cat. no. BBA20; 1:300; R&D Systems, Inc.) antibodies or the isotype controls (cat. no. MAB0031; 1:200; R&D Systems, Inc.) at 37°C for 30 min, rinsed twice with PBS and fixed in 10% (v/v) formaldehyde and PBS at 37°C for 25 min. Then, the cells were sorted and observed using a BD FACSCalibur 4-color flow cytometer (BD Biosciences).

Techniques: Expressing, Flow Cytometry, Fluorescence, Small Interfering RNA